The development of graphene quantum dots (GQDs) with low toxicity, excellent dis-persibility, and high photostability has led to extensive progress in bio-imaging and optical sensing applications. However, one-pot synthesis and mass production of GQDs, and tuning their photolu-minescence, remains a challenge. Here we demonstrate a simple and scalable method for fabricating GQDs with high size uniformity and chemical stability, via a sequential process of inserting alkali metal into graphite (Stage I: KC8 and Stage II: KC24 ) and exfoliation to GQDs in a selected solvent. Structural and optical measurements were conducted, and the emitted colors of the as-prepared GQDs were blue (KC8 ) and yellow (KC24 ), respectively. The stage of graphite intercalation in the compounds played an important role in the size and thickness of the GQD. The as-prepared GQDs had clear char- acteristic peaks consistent with the quantum confinement effect and intrinsic/extrinsic states. Our approach will provide great potential for a wide variety of bioimaging and bioanalysis applications.